An Improved Method for Using Electrolyte Leakage to Assess Membrane Competence in Plant Tissues

An improved HPLC method for determination of colocynthin in colocynth

Background and objectives: Colocynthin is the major active secondary metabolite of colocynth, Citrullus colocynthis (L.) Schrad, which has been used in traditional and ethno medicine of many countries.  It could be considered as an active marker for quality control of colocynth and its herbal products. Analysis and standardization of colocynth and its herbal preparations are a critical...

Modelling and Compensation of uncertain time-delays in networked control systems with plant uncertainty using an Improved RMPC Method

Control systems with digital communication between sensors, controllers and actuators are called as Networked Control Systems (NCSs). In general, NCSs encounter with some problems such as packet dropouts and network induced delays. When plant uncertainty is added to the aforementioned problems, the design of the robust controller that is able to guarantee the stability, becomes more complex. In...

An improved method for handling tissues for immunofluorescence.

screening potato cultivars and wild species to abiotic stresses using an electrolyte leakage bioassay

ten solanum tuberosum cultivars and accessions from 11 wild solanum species were evaluated for their tolerance to salt (200 mm nacl or 100 mm na2so4), drought (35% peg), cold (-4ºc) and heat (37ºc). evaluation was based on electrolyte leakage from de-tached leaves of in vitro plantlets. testing involved a rinsing treatment with three changes of distilled water to remove electrolytes from leaf a...

Using Electrolyte Leakage for Evaluating Hardwood Seedling Cold Hardiness

This paper describes the use of electrolyte leakage (EL) from stem tissue as a potential method for assessing cold hardiness of hardwood seedlings. The EL method has seen little use with North American hardwoods, but has successfully predicted conifer hardiness in both controlled and operational settings and has been used experimentally on hardwoods in Europe. Three species of hardwoods—norther...